| 1 | Florida Agency for Health Care Administration · Tallahassee | State and local governments | 21 | $111 billion |
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| 2 | Florida Department of Education · Tallahassee | State and local governments | 157 | $29 billion |
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| 3 | State of Florida Department of Transportation · Tallahassee | State and local governments | 10,518 | $15 billion |
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| 4 | State of Florida Division of Emergency Management · Tallahassee | State and local governments | 115 | $12 billion |
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| 5 | Florida Department of Children and Families · Tallahassee | State and local governments | 188 | $9.9 billion |
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| 6 | Department of Agriculture & Consumer Services Florida · Tallahassee | State and local governments | 280 | $8.5 billion |
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| 7 | Florida Department of Health · Tallahassee | State and local governments | 271 | $7.8 billion |
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| 8 | Florida Department of Commerce · Tallahassee | State and local governments | 134 | $7.5 billion |
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| 9 | University of Florida · Gainesville | Colleges and universities | 2,849 | $2.5 billion |
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| 10 | Florida Department of Environmental Protection · Tallahassee | State and local governments | 207 | $2.0 billion |
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| 11 | County of Lee · Fort Myers | State and local governments | 94 | $1.3 billion |
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| 12 | County of Miami-Dade · Miami | State and local governments | 33 | $1.1 billion |
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| 13 | University of South Florida · Tampa | Colleges and universities | 734 | $1.1 billion |
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| 14 | State of Florida Department of Revenue · Tallahassee | State and local governments | 6 | $999 million |
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| 15 | Broward, County of · Fort Lauderdale | State and local governments | 204 | $949 million |
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| 16 | County of Pinellas · Clearwater | State and local governments | 75 | $925 million |
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| 17 | Florida State University · Tallahassee | Colleges and universities | 734 | $917 million |
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| 18 | University of Miami · Miami | Colleges and universities | 634 | $903 million |
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| 19 | Florida International University · Miami | Colleges and universities | 740 | $827 million |
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| 20 | Department of Elder Affairs Florida · Tallahassee | State and local governments | 104 | $799 million |
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| 21 | The University of Central Florida Board of Trustees · Orlando | Colleges and universities | 706 | $766 million |
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| 22 | The State of Florida Judiciary Courts · Tallahassee | State and local governments | 17 | $687 million |
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| 23 | Pasco County Board of County Commissioners · New Port Richey | State and local governments | 45 | $650 million |
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| 24 | Greater Orlando Aviation Authority · Orlando | State and local governments | 30 | $596 million |
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| 25 | County of Volusia · Deland | State and local governments | 73 | $595 million |
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| 26 | Miami-Dade County · Miami | State and local governments | 48 | $538 million |
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| 27 | Florida a & M University · Tallahassee | Colleges and universities | 335 | $533 million |
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| 28 | County of Miami-Dade · Miami | State and local governments | 274 | $531 million |
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| 29 | Sarasota, County of · Sarasota | State and local governments | 54 | $500 million |
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| 30 | Orange County, Florida · Orlando | State and local governments | 64 | $494 million |
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| 31 | Dade County Aviation · Miami | State and local governments | 38 | $473 million |
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| 32 | State of Florida Governors Office · Tallahassee | State and local governments | 2 | $434 million |
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| 33 | Hillsborough County of · Tampa | State and local governments | 51 | $410 million |
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| 34 | County of Palm Beach · West Palm Beach | State and local governments | 110 | $406 million |
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| 35 | Department of Legal Affairs Florida · Tallahassee | State and local governments | 10 | $405 million |
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| 36 | State of Florida Department of Financial Services · Tallahassee | State and local governments | 2 | $366 million |
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| 37 | Florida Department of Veterans Affairs · Port Saint Lucie | State and local governments | 9 | $365 million |
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| 38 | Central Florida Regional Transportation Authority · Orlando | Other | 27 | $363 million |
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| 39 | Department of Military Florida · Saint Augustine | State and local governments | 93 | $354 million |
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| 40 | Lutheran Services Florida, Inc. · Tampa | Nonprofits | 30 | $351 million |
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| 41 | Florida Department of Transportation · Pompano Beach | State and local governments | 18 | $349 million |
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| 42 | Seminole Electric Cooperative, Inc · Tampa | Nonprofits | 3 | $332 million |
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| 43 | H. Lee Moffitt Cancer Center and Research Institute Hospital, Inc. · Tampa | Nonprofits | 246 | $332 million |
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| 44 | Miami Dade College · Miami | Colleges and universities | 31 | $326 million |
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| 45 | Redlands Christian Migrant Association, Inc · Immokalee | Nonprofits | 14 | $308 million |
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| 46 | Mayo Clinic Jacksonville (a Nonprofit Corporation) · Jacksonville | Nonprofits | 150 | $308 million |
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| 47 | University of Miami · Key Biscayne | Colleges and universities | 189 | $290 million |
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| 48 | Florida Housing Finance Corporation · Tallahassee | State and local governments | 11 | $275 million |
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| 49 | Florida Atlantic University · Boca Raton | Colleges and universities | 240 | $272 million |
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| 50 | Hillsborough County Aviation Authority · Tampa | State and local governments | 27 | $254 million |
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| 51 | Pinellas Suncoast Transit Authority · Saint Petersburg | State and local governments | 33 | $242 million |
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| 52 | Jacksonville Transportation Authority · Jacksonville | Other | 35 | $241 million |
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| 53 | Episcopal Childrens Services Inc · Jacksonville | Nonprofits | 10 | $225 million |
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| 54 | Florida Fish and Wildlife Conservation Commission · Tallahassee | State and local governments | 118 | $223 million |
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| 55 | Broward College · Fort Lauderdale | State and local governments | 44 | $222 million |
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| 56 | Miami-Dade County Department of Cultural Affairs · Miami | State and local governments | 235 | $212 million |
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| 57 | Hillsborough Transit Authority · Tampa | Other | 24 | $208 million |
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| 58 | Enersys Advanced Systems Inc · Pinellas Park | Businesses | 1 | $199 million |
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| 59 | Vincentian Cares, Inc. · Saint Petersburg | Nonprofits | 71 | $196 million |
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| 60 | The Institute for Intergovernmental Research, Inc. · Tallahassee | Nonprofits | 45 | $195 million |
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| 61 | City of Miami · Miami | State and local governments | 59 | $189 million |
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| 62 | City of Jacksonville, Florida · Jacksonville | State and local governments | 93 | $180 million |
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| 63 | Valencia College · Orlando | State and local governments | 5 | $172 million |
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| 64 | University of Miami · Miami | Colleges and universities | 239 | $169 million |
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| 65 | City of Tallahassee · Tallahassee | State and local governments | 64 | $169 million |
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| 66 | Solar and Energy Loan Fund of St. Lucie County, Inc. · Fort Pierce | Nonprofits | 3 | $158 million |
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| 67 | Florida Commission on Community Service · Tallahassee | State and local governments | 23 | $156 million |
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| 68 | The School Board of Broward County, Florida · Fort Lauderdale | State and local governments | 23 | $153 million |
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| 69 | Palm Beach State College · Lake Worth | State and local governments | 22 | $145 million |
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| 70 | Miami-Dade, County of · Miami | Other | 4 | $142 million |
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| 71 | Community Health of South Florida, Inc. · Miami | Nonprofits | 22 | $130 million |
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| 72 | Lee County Port Authority · Fort Myers | State and local governments | 17 | $130 million |
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| 73 | Key West International Airport · Key West | State and local governments | 32 | $128 million |
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| 74 | City of Tampa · Tampa | State and local governments | 92 | $126 million |
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| 75 | Acuity-CHS, LLC · Cape Canaveral | Businesses | 6 | $124 million |
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| 76 | Hillsborough County School District · Tampa | State and local governments | 29 | $122 million |
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| 77 | Everglades College Inc · Fort Lauderdale | Businesses | 3 | $121 million |
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| 78 | County of Monroe · Key West | State and local governments | 20 | $118 million |
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| 79 | Gainesville, City of · Gainesville | State and local governments | 40 | $112 million |
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| 80 | The Gulf Consortium · Winter Park | State and local governments | 33 | $111 million |
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| 81 | University of West Florida · Pensacola | Colleges and universities | 60 | $109 million |
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| 82 | Embry-Riddle Aeronautical University, Inc. · Daytona Beach | Colleges and universities | 185 | $109 million |
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| 83 | Hillsborough College · Tampa | Colleges and universities | 13 | $108 million |
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| 84 | School Board of Miami-Dade County · Miami | State and local governments | 28 | $106 million |
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| 85 | Florida State College at Jacksonville · Jacksonville | Colleges and universities | 22 | $105 million |
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| 86 | Florida Department of Highway Safety and Motor Vehicles · Tallahassee | State and local governments | 11 | $105 million |
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| 87 | Nova Southeastern University, Inc · Fort Lauderdale | Colleges and universities | 90 | $104 million |
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| 88 | Florida Department of Law Enforcement · Tallahassee | State and local governments | 70 | $104 million |
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| 89 | Sarasota Manatee Airport Authority · Sarasota | State and local governments | 26 | $97.4 million |
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| 90 | Homeless Services Network of Central Florida, Inc. · Orlando | Nonprofits | 106 | $94.0 million |
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| 91 | Jacksonville Housing Authority · Jacksonville | Tribal governments and organizations | 103 | $93.9 million |
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| 92 | Florida Gulf Coast University · Fort Myers | Colleges and universities | 45 | $93.8 million |
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| 93 | Jacksonville Aviation Authority · Jacksonville | State and local governments | 26 | $92.2 million |
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| 94 | Board of Trustees, St. Petersburg College · Clearwater | State and local governments | 16 | $90.8 million |
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| 95 | Mid Florida Community Services, Inc. · Brooksville | Nonprofits | 8 | $89.7 million |
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| 96 | Charlotte County Airport Authority · Punta Gorda | State and local governments | 14 | $87.8 million |
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| 97 | Bethune-Cookman University, Inc. · Daytona Beach | Colleges and universities | 25 | $87.5 million |
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| 98 | Jaeb Center for Health Research Foundation, Inc. · Tampa | Nonprofits | 7 | $85.4 million |
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| 99 | County of Collier · Naples | State and local governments | 58 | $84.8 million |
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| 100 | Indian River State College · Fort Pierce | Colleges and universities | 30 | $84.3 million |
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