| 1 | Finance & Administration Tennessee Depar · Nashville | State and local governments | 22 | $50 billion |
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| 2 | Department of Education Tennessee · Nashville | State and local governments | 139 | $10 billion |
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| 3 | Tennessee Department of Transportation · Nashville | State and local governments | 5,361 | $6.9 billion |
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| 4 | Tennessee Department of Human Services · Nashville | State and local governments | 154 | $5.5 billion |
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| 5 | Vanderbilt University Medical Center · Nashville | Nonprofits | 1,186 | $2.2 billion |
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| 6 | Tennessee Department of Health · Nashville | State and local governments | 227 | $2.2 billion |
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| 7 | Tennessee Emergency Management Authority · Nashville | State and local governments | 86 | $1.3 billion |
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| 8 | Department of Environment & Conservation Tennessee · Nashville | State and local governments | 178 | $1.3 billion |
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| 9 | Economic and Community Develop · Nashville | State and local governments | 39 | $1.2 billion |
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| 10 | Vanderbilt University Medical Center · Nashville | Colleges and universities | 1,215 | $1.0 billion |
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| 11 | Department of Childrens Services Tennessee · Nashville | State and local governments | 77 | $795 million |
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| 12 | TN Department of Labor & Workforce Dr · Nashville | State and local governments | 112 | $676 million |
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| 13 | Tennessee Housing Development Agency · Nashville | State and local governments | 38 | $664 million |
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| 14 | Tennessee Department of Finance and Administration · Nashville | State and local governments | 84 | $584 million |
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| 15 | Tennessee Department of Mental Health & Substance Abuse Services · Nashville | State and local governments | 56 | $580 million |
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| 16 | University of Tennessee · Knoxville | Colleges and universities | 636 | $549 million |
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| 17 | St Jude Children's Research Hospital Inc · Memphis | Nonprofits | 291 | $506 million |
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| 18 | Eastman Chemical Company · Kingsport | Businesses | 1 | $375 million |
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| 19 | Tennessee State University · Nashville | Colleges and universities | 226 | $358 million |
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| 20 | University of Tennessee · Memphis | Colleges and universities | 244 | $305 million |
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| 21 | Memphis-Shelby County Airport Authority · Memphis | State and local governments | 18 | $287 million |
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| 22 | TN State Veterans Home · Humboldt | State and local governments | 14 | $263 million |
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| 23 | Tennessee Department of Military · Nashville | State and local governments | 91 | $262 million |
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| 24 | Meharry Medical College · Nashville | Colleges and universities | 100 | $255 million |
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| 25 | Memphis Area Transit Authority · Memphis | Other | 23 | $238 million |
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| 26 | Nashville & Davidson County, Metropolitan Government of · Nashville | State and local governments | 78 | $230 million |
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| 27 | Wildlife Resources Agency, Tennessee · Nashville | State and local governments | 69 | $217 million |
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| 28 | Tennessee Commission on Aging and Disability · Nashville | State and local governments | 75 | $207 million |
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| 29 | University of Memphis · Memphis | Colleges and universities | 198 | $195 million |
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| 30 | Safety & Homeland Security · Nashville | State and local governments | 72 | $182 million |
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| 31 | Nashville Metropolitan Transit Authority · Madison | State and local governments | 20 | $174 million |
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| 32 | City of Memphis, Tennessee · Memphis | State and local governments | 70 | $173 million |
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| 33 | Metropolitan Nashville Airport Authority (the) · Nashville | State and local governments | 17 | $171 million |
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| 34 | University of Tennessee · Knoxville | Colleges and universities | 55 | $162 million |
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| 35 | East Tennessee State University · Johnson City | Colleges and universities | 132 | $153 million |
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| 36 | Shelby County Board of Education · Memphis | State and local governments | 14 | $145 million |
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| 37 | Middle Tennessee State University · Murfreesboro | Colleges and universities | 99 | $140 million |
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| 38 | City of Chattanooga · Chattanooga | State and local governments | 51 | $128 million |
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| 39 | Chattanooga Housing Authority · Chattanooga | State and local governments | 71 | $126 million |
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| 40 | Tennessee Technological University · Cookeville | Colleges and universities | 107 | $112 million |
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| 41 | City of Knoxville · Knoxville | State and local governments | 65 | $110 million |
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| 42 | Shelby County Government · Memphis | State and local governments | 54 | $102 million |
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| 43 | Novonix Anode Materials LLC · Chattanooga | Businesses | 1 | $100 million |
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| 44 | MP Assets Corporation · Piney Flats | Businesses | 1 | $100 million |
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| 45 | Family Resource Agency Inc · Cleveland | Nonprofits | 5 | $97.4 million |
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| 46 | Department of Agriculture Tennessee · Nashville | State and local governments | 172 | $97.4 million |
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| 47 | University of Tennessee · Knoxville | Colleges and universities | 165 | $97.3 million |
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| 48 | Delek Us Holdings, Inc · Brentwood | Businesses | 1 | $95.0 million |
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| 49 | U.S. Cotton Trust Protocol, LLC · Cordova | Nonprofits | 1 | $90.5 million |
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| 50 | Catholic Charities of Tennessee Inc · Nashville | Nonprofits | 14 | $90.1 million |
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| 51 | Oak Ridge Associated Universities, Incorporated · Oak Ridge | Nonprofits | 524 | $88.6 million |
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| 52 | University of Tennessee · Chattanooga | Colleges and universities | 70 | $84.5 million |
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| 53 | Knoxville Knox County Community Action Committee · Knoxville | State and local governments | 44 | $80.3 million |
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| 54 | Austin Peay State University · Clarksville | Colleges and universities | 38 | $77.7 million |
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| 55 | Porter-Leath · Memphis | Nonprofits | 15 | $76.6 million |
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| 56 | State of Tennessee · Nashville | State and local governments | 12 | $72.3 million |
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| 57 | Metropolitan Knoxville Airport Authority (the) (" ") · Alcoa | State and local governments | 14 | $71.1 million |
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| 58 | Cherokee Health Systems · Knoxville | Nonprofits | 13 | $69.2 million |
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| 59 | South Central Human Resource Agency, Inc. · Fayetteville | Nonprofits | 10 | $66.9 million |
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| 60 | Douglas-Cherokee Economic Authority, Inc. · Morristown | Nonprofits | 11 | $66.7 million |
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| 61 | Memphis Housing Authority · Memphis | State and local governments | 222 | $64.3 million |
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| 62 | Tennessee Bureau of Investigation · Nashville | State and local governments | 32 | $63.8 million |
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| 63 | Upper East Tennessee Human Development Agency, Inc. · Kingsport | Nonprofits | 5 | $62.5 million |
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| 64 | North West Tennessee Economic Development Council · Dresden | Nonprofits | 2 | $62.1 million |
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| 65 | LBJ & C Development Corp · Cookeville | Nonprofits | 8 | $60.5 million |
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| 66 | United Neighborhood Health Services, Inc. · Nashville | Nonprofits | 7 | $60.4 million |
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| 67 | Mid-Cumberland Community Action Agency, Inc. · Mount Juliet | Nonprofits | 5 | $60.3 million |
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| 68 | Knoxvilles Community Development Corp · Knoxville | State and local governments | 23 | $60.3 million |
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| 69 | United South & Eastern Tribes Inc · Nashville | Tribal governments and organizations | 43 | $59.5 million |
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| 70 | Fisk University · Nashville | Colleges and universities | 35 | $58.5 million |
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| 71 | Chattanooga Area Regional Transportation Authority · Chattanooga | State and local governments | 17 | $57.6 million |
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| 72 | Southwest TN Community College · Memphis | Colleges and universities | 10 | $57.1 million |
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| 73 | Chattanooga Metropolitan Airport Authority · Chattanooga | State and local governments | 15 | $52.9 million |
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| 74 | Christ Community Health Services Inc · Memphis | Other | 8 | $51.8 million |
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| 75 | Lane College · Jackson | Colleges and universities | 11 | $48.0 million |
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| 76 | Metropolitan Nashville Public Schools · Nashville | State and local governments | 13 | $43.3 million |
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| 77 | Centerstone of Tennessee, Inc. · Nashville | Nonprofits | 22 | $42.5 million |
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| 78 | Volunteer Tennessee · Nashville | State and local governments | 16 | $37.5 million |
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| 79 | Regional Organized Crime Information Center · Nashville | Nonprofits | 2 | $36.7 million |
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| 80 | Matthew Walker Comprehensive Health Center, Inc. · Nashville | Other | 8 | $36.4 million |
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| 81 | Electric Power Board of Chattanooga · Chattanooga | State and local governments | 2 | $36.3 million |
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| 82 | Chattanooga State Community College · Chattanooga | State and local governments | 8 | $36.1 million |
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| 83 | Chickasaw Electric Cooperative · Somerville | Other | 1 | $35.7 million |
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| 84 | Northeast State Community College · Blountville | Nonprofits | 18 | $35.1 million |
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| 85 | Regional Transportation Auth · Madison | Other | 10 | $34.4 million |
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| 86 | Lafollette Housing Authority · La Follette | State and local governments | 29 | $33.9 million |
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| 87 | Memphis Health Center Inc · Memphis | Other | 7 | $33.5 million |
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| 88 | Sequatchie Valley Planning & Development Agency · Jasper | Nonprofits | 6 | $31.6 million |
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| 89 | Tri-Cities Airport Authority · Blountville | State and local governments | 18 | $31.2 million |
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| 90 | Rural Health Services Consortium, Inc · Rogersville | Other | 6 | $31.0 million |
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| 91 | Meharry Medical College · Nashville | Colleges and universities | 1 | $30.6 million |
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| 92 | Clinch-Powell Educational Cooperative · Tazewell | Nonprofits | 3 | $30.4 million |
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| 93 | Murfreesboro, City of · Murfreesboro | State and local governments | 32 | $30.3 million |
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| 94 | Nashville State Community College · Nashville | Colleges and universities | 3 | $29.8 million |
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| 95 | University of Tennessee · Martin | Colleges and universities | 27 | $29.8 million |
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| 96 | Volunteer State Community College · Gallatin | Colleges and universities | 8 | $29.7 million |
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| 97 | Jackson Housing Authority · Jackson | Tribal governments and organizations | 60 | $28.9 million |
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| 98 | Lemoyne-Owen College · Memphis | Colleges and universities | 8 | $28.3 million |
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| 99 | Southwest Human Resource Agency · Henderson | Nonprofits | 8 | $28.1 million |
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| 100 | American Baptist Theological Seminary (the) · Nashville | Nonprofits | 8 | $26.2 million |
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