| 1 | NYS Department of Health · Albany | State and local governments | 200 | $310 billion |
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| 2 | New York State Education Department · Albany | State and local governments | 183 | $36 billion |
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| 3 | Metropolitan Transportation Authority · New York | State and local governments | 83 | $27 billion |
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| 4 | New York State Office of Temporary & Disability Assistance · Albany | State and local governments | 116 | $21 billion |
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| 5 | New York State Division of Homeland Security & Emergency Services · Albany | State and local governments | 110 | $19 billion |
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| 6 | Department of Transportation New York · Albany | State and local governments | 7,489 | $13 billion |
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| 7 | New York City Housing Authority · New York | State and local governments | 941 | $10 billion |
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| 8 | Children & Family Services, New York Office of · Rensselaer | State and local governments | 134 | $8.9 billion |
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| 9 | Gateway Development Commission · New York | State and local governments | 3 | $4.4 billion |
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| 10 | The Trustees of Columbia University in the City of New York · New York | Colleges and universities | 2,183 | $3.9 billion |
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| 11 | Department of Labor New York · Albany | State and local governments | 118 | $3.0 billion |
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| 12 | Icahn School of Medicine at Mount Sinai · New York | Colleges and universities | 1,496 | $2.3 billion |
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| 13 | Department of Environmental Conservation New York · Albany | State and local governments | 187 | $2.2 billion |
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| 14 | City of New York · Long Island City | State and local governments | 61 | $2.1 billion |
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| 15 | Health Research, Inc. · Albany | State and local governments | 64 | $2.1 billion |
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| 16 | New York University · New York | Colleges and universities | 1,261 | $2.0 billion |
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| 17 | Inclusiv, Inc · New York | Nonprofits | 5 | $1.9 billion |
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| 18 | Cornell University · Ithaca | Colleges and universities | 1,989 | $1.8 billion |
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| 19 | University of Rochester · Rochester | Colleges and universities | 1,196 | $1.7 billion |
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| 20 | International Rescue Committee, Inc. · New York | Nonprofits | 218 | $1.5 billion |
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| 21 | Weill Medical College of Cornell University · New York | Colleges and universities | 1,111 | $1.5 billion |
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| 22 | City of New York - Mayors Office of Criminal Justice · New York | State and local governments | 23 | $1.5 billion |
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| 23 | Port Authority of New York & New Jersey · New York | State and local governments | 59 | $1.4 billion |
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| 24 | The City University of New York · New York | State and local governments | 4 | $1.3 billion |
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| 25 | The Trustees of Columbia University in the City of New York · New York | Colleges and universities | 1,369 | $1.2 billion |
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| 26 | Sloan-Kettering Institute for Cancer Research · New York | Nonprofits | 650 | $1.2 billion |
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| 27 | New York State Urban Development Corporation · New York | State and local governments | 10 | $1.1 billion |
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| 28 | The Mental Health Association of New York City, Inc. · New York | Nonprofits | 8 | $1.1 billion |
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| 29 | New York City Office of Management & Budget · New York | State and local governments | 9 | $1.1 billion |
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| 30 | Albert Einstein College of Medicine · Bronx | Colleges and universities | 666 | $964 million |
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| 31 | New York University · New York | Colleges and universities | 1,124 | $914 million |
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| 32 | Church World Service, Inc. · New York | Nonprofits | 60 | $878 million |
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| 33 | Institute of International Education Inc · New York | Nonprofits | 114 | $832 million |
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| 34 | The Research Foundation for the State University of New York · Stony Brook | Nonprofits | 985 | $798 million |
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| 35 | Addiction Services & Supports New York State Office of · Albany | State and local governments | 9 | $769 million |
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| 36 | The Research Foundation for the State University of New York · Buffalo | Colleges and universities | 921 | $712 million |
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| 37 | New York State Energy Research & Development Authority · Albany | State and local governments | 20 | $691 million |
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| 38 | New York State Office for the Aging · Albany | State and local governments | 74 | $664 million |
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| 39 | NYS Division Housing & Comm Renewal · Albany | State and local governments | 8 | $602 million |
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| 40 | City of New York Housing Preservation & Development · New York | State and local governments | 232 | $547 million |
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| 41 | Niagara Frontier Transportation Authority · Buffalo | State and local governments | 73 | $546 million |
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| 42 | State of New York · Albany | State and local governments | 9 | $508 million |
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| 43 | Acelero Inc · New York | Businesses | 21 | $471 million |
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| 44 | City of New York Board of Education · Brooklyn | State and local governments | 37 | $462 million |
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| 45 | Cayuga Home for Children · Auburn | Nonprofits | 8 | $456 million |
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| 46 | County of Westchester · White Plains | State and local governments | 139 | $450 million |
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| 47 | New York State Division of Military & Naval Affairs · Latham | State and local governments | 86 | $424 million |
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| 48 | New York State Office of Mental Health · Albany | State and local governments | 17 | $422 million |
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| 49 | New York City Department of Transportation · New York | State and local governments | 52 | $398 million |
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| 50 | The Rockefeller University · New York | Colleges and universities | 252 | $386 million |
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| 51 | Housing Trust Fund Corp · Albany | State and local governments | 15 | $373 million |
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| 52 | Department of State New York · Albany | State and local governments | 18 | $352 million |
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| 53 | NYS Office of Victim Services · Albany | State and local governments | 10 | $343 million |
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| 54 | Public Health Solutions · New York | Nonprofits | 18 | $333 million |
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| 55 | Capital District Transportation Authority · Albany | Other | 29 | $306 million |
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| 56 | Research Foundation for Mental Hygiene, Inc. · Albany | Nonprofits | 8 | $294 million |
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| 57 | Unicef · New York | Nonprofits | 34 | $275 million |
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| 58 | Syracuse University · Syracuse | Colleges and universities | 390 | $262 million |
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| 59 | Research Foundation for Mental Hygiene, Inc. · New York | Nonprofits | 208 | $259 million |
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| 60 | New York State Housing Finance Agency · New York | Nonprofits | 11 | $253 million |
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| 61 | Health Research, Inc. · Buffalo | Nonprofits | 181 | $248 million |
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| 62 | Cold Spring Harbor Laboratory · Cold Spring Harbor | Businesses | 215 | $235 million |
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| 63 | The Research Foundation for the State University of New York · Binghamton | Nonprofits | 271 | $234 million |
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| 64 | The American University in Cairo · New York | Colleges and universities | 39 | $228 million |
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| 65 | Nassau, County of · Mineola | State and local governments | 14 | $217 million |
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| 66 | Attorney General, New York State · New York | State and local governments | 5 | $213 million |
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| 67 | Rising Ground, Inc · New York | Nonprofits | 22 | $211 million |
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| 68 | Research Foundation of the City University of New York · New York | Colleges and universities | 253 | $199 million |
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| 69 | Rochester Institute of Technology · Rochester | Colleges and universities | 361 | $198 million |
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| 70 | Rochester Genesee Regional Transportation Authority · Rochester | State and local governments | 17 | $198 million |
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| 71 | Local Initiatives Support Corporation · Washington | Nonprofits | 64 | $197 million |
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| 72 | The Feinstein Institutes for Medical Research · Manhasset | Nonprofits | 136 | $196 million |
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| 73 | Ge Vernova Operations LLC · Schenectady | Businesses | 30 | $187 million |
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| 74 | Yeled V'Yalda Early Childhood Center Inc. · Brooklyn | Nonprofits | 4 | $187 million |
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| 75 | Rensselaer Polytechnic Institute · Troy | Colleges and universities | 308 | $185 million |
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| 76 | Domestic and Foreign Missionary Society of the Protestant Episcopal Church in the USA · New York | Nonprofits | 19 | $184 million |
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| 77 | Sun River Health, Inc. · Peekskill | Nonprofits | 24 | $184 million |
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| 78 | The Research Foundation for the State University of New York · Albany | Nonprofits | 295 | $183 million |
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| 79 | Buffalo Municipal Housing Auth · Buffalo | State and local governments | 145 | $179 million |
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| 80 | Concern Worldwide Us Inc · New York | Nonprofits | 32 | $173 million |
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| 81 | Veterans Affairs New York Division of · Stony Brook | State and local governments | 14 | $171 million |
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| 82 | Department of Motor Vehicles New York State · Albany | State and local governments | 60 | $166 million |
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| 83 | Sunset Park Health Council, Inc. · Brooklyn | Nonprofits | 38 | $164 million |
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| 84 | The Research Foundation for the State University of New York · Brooklyn | Nonprofits | 106 | $162 million |
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| 85 | Health Research, Inc. · Albany | State and local governments | 55 | $160 million |
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| 86 | The Research Foundation for the State University of New York · Albany | Colleges and universities | 30 | $155 million |
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| 87 | Syracuse Housing Authority · Syracuse | State and local governments | 58 | $153 million |
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| 88 | Suffolk, County of · Yaphank | State and local governments | 17 | $150 million |
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| 89 | United Nations Development Programme · New York | Nonprofits | 28 | $146 million |
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| 90 | National Urban League, Inc. · New York | Nonprofits | 35 | $145 million |
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| 91 | The Research Foundation for the State University of New York · Syracuse | Nonprofits | 137 | $137 million |
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| 92 | City of Buffalo · Buffalo | State and local governments | 37 | $137 million |
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| 93 | The Community Action Organization of Western New York, Inc. · Buffalo | Nonprofits | 7 | $135 million |
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| 94 | New York State Division of Criminal Justice Services · Albany | State and local governments | 47 | $134 million |
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| 95 | Center for Internet Security, Inc. · East Greenbush | Nonprofits | 2 | $134 million |
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| 96 | Corning Incorporated · Corning | Businesses | 2 | $134 million |
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| 97 | Wildlife Conservation Society · Bronx | Nonprofits | 125 | $134 million |
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| 98 | Fordham University · Bronx | Colleges and universities | 69 | $132 million |
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| 99 | The New York Foundling · New York | Nonprofits | 13 | $131 million |
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| 100 | Catholic Charities Neighborhood Services, Inc · Brooklyn | Nonprofits | 15 | $128 million |
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