| 1 | Department of Social Services Misso · Jefferson City | State and local governments | 231 | $62 billion |
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| 2 | Elementary & Secondary Education Missouri Department of · Jefferson City | State and local governments | 173 | $10.0 billion |
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| 3 | Missouri Department of Transportation · Jefferson City | State and local governments | 7,179 | $7.6 billion |
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| 4 | The Washington University · Saint Louis | Colleges and universities | 2,793 | $3.6 billion |
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| 5 | Missouri Department of Health & Senior Services · Jefferson City | State and local governments | 301 | $2.5 billion |
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| 6 | Missouri Department of Economic Development · Jefferson City | State and local governments | 7 | $1.8 billion |
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| 7 | Missouri Department of Natural R · Jefferson City | State and local governments | 252 | $1.2 billion |
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| 8 | Missouri Department of Public Safety · Jefferson City | State and local governments | 60 | $1.2 billion |
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| 9 | University of Missouri System · Columbia | Colleges and universities | 1,088 | $1.1 billion |
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| 10 | The Bi State Development Agency of the Mo IL Metro Dist · Saint Louis | State and local governments | 41 | $887 million |
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| 11 | Missouri Department of Mental Health · Jefferson City | State and local governments | 181 | $549 million |
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| 12 | Department of Economic Development Missouri · Jefferson City | State and local governments | 45 | $471 million |
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| 13 | City of Kansas City · Kansas City | State and local governments | 69 | $369 million |
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| 14 | Veterans Commission Missouri · Jefferson City | State and local governments | 24 | $361 million |
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| 15 | State of Missouri · Jefferson City | State and local governments | 6 | $357 million |
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| 16 | Missouri Department of Elementary and Secondary Education · Jefferson City | State and local governments | 21 | $349 million |
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| 17 | Kansas City Area Transportation Authority · Kansas City | State and local governments | 43 | $319 million |
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| 18 | Missouri Department of Labor and Industrial Relations · Jefferson City | State and local governments | 28 | $275 million |
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| 19 | City of St. Louis · Saint Louis | State and local governments | 29 | $251 million |
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| 20 | Missouri State University · Springfield | Colleges and universities | 77 | $235 million |
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| 21 | Missouri Department of Higher Education · Jefferson City | State and local governments | 55 | $225 million |
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| 22 | Missouri Department of Conservation · Jefferson City | State and local governments | 114 | $221 million |
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| 23 | The Curators of the University of Missouri · Kansas City | Colleges and universities | 161 | $214 million |
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| 24 | The City of St Louis · Saint Louis | State and local governments | 31 | $207 million |
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| 25 | Icl Specialty Products Inc · Saint Louis | Businesses | 1 | $197 million |
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| 26 | Saint Louis University · Saint Louis | Colleges and universities | 219 | $194 million |
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| 27 | Department of Public Safety Missouri · Jefferson City | State and local governments | 60 | $179 million |
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| 28 | Mid America Regional Council · Kansas City | Nonprofits | 24 | $174 million |
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| 29 | University of Missouri System · Columbia | Colleges and universities | 55 | $173 million |
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| 30 | St. Louis County, Missouri · Saint Louis | State and local governments | 90 | $164 million |
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| 31 | State Environmental Improvement & Energy Resources Authority · Jefferson City | State and local governments | 2 | $157 million |
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| 32 | Lincoln University · Jefferson City | Colleges and universities | 90 | $156 million |
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| 33 | Department of Public Safety Missouri · Jefferson City | State and local governments | 45 | $153 million |
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| 34 | University of Missouri System · Rolla | Colleges and universities | 230 | $134 million |
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| 35 | Kansas City, Missouri, City of · Kansas City | State and local governments | 21 | $125 million |
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| 36 | St. Louis Housing Authority · Saint Louis | State and local governments | 206 | $117 million |
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| 37 | Young Women's Christian Association of Metropolitan St. Louis, Missouri · Saint Louis | Nonprofits | 2 | $108 million |
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| 38 | Waynesville R-6 School District · Waynesville | State and local governments | 9 | $106 million |
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| 39 | International Institute of Metropolitan St. Louis · Saint Louis | Nonprofits | 20 | $105 million |
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| 40 | Childrens Mercy Hospital · Kansas City | Nonprofits | 71 | $100 million |
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| 41 | Youth in Need · Saint Charles | Nonprofits | 19 | $97.1 million |
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| 42 | Ozarks Area Community Action Corporation · Springfield | Nonprofits | 6 | $94.3 million |
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| 43 | Delta Area Economic Opportunity Corporation · Sikeston | Nonprofits | 16 | $83.8 million |
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| 44 | Donald Danforth Plant Science Center · Saint Louis | Nonprofits | 77 | $81.8 million |
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| 45 | Roeslein Alternative Energy, LLC · Saint Louis | Businesses | 1 | $80.0 million |
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| 46 | North Central Missouri Regional Water Commission · Milan | State and local governments | 6 | $78.5 million |
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| 47 | Compass Health, Inc · Clinton | Nonprofits | 20 | $77.1 million |
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| 48 | Junior College District of St Louis · Bridgeton | Colleges and universities | 20 | $73.4 million |
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| 49 | Swope Health Services · Kansas City | Nonprofits | 15 | $72.2 million |
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| 50 | Housing Authority of Kansas City, MO · Kansas City | Tribal governments and organizations | 126 | $72.0 million |
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| 51 | Urban League of Metropolitan St. Louis · Saint Louis | Nonprofits | 10 | $69.5 million |
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| 52 | Missouri National Guard · Jefferson City | State and local governments | 23 | $68.1 million |
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| 53 | Harris-Stowe State University · Saint Louis | Colleges and universities | 23 | $67.4 million |
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| 54 | University of Missouri System · Saint Louis | Colleges and universities | 54 | $67.3 million |
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| 55 | The Boeing Company · Saint Louis | Businesses | 19 | $66.0 million |
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| 56 | Kansas City Downtown Airport - Wheeler Field · Kansas City | State and local governments | 10 | $64.4 million |
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| 57 | Ozarks Tech Community College · Springfield | State and local governments | 14 | $63.8 million |
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| 58 | Junior College District of Metropolitan · Kansas City | State and local governments | 8 | $61.5 million |
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| 59 | Economic Security Corporation of Southwest Area · Joplin | Nonprofits | 40 | $58.9 million |
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| 60 | Missouri Housing Development Commission · Kansas City | State and local governments | 11 | $58.3 million |
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| 61 | City Utilities of Springfield · Springfield | State and local governments | 18 | $57.9 million |
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| 62 | Advocates for a Healthy Community Inc · Springfield | Other | 15 | $57.9 million |
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| 63 | Affinia Healthcare · Saint Louis | Other | 9 | $57.6 million |
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| 64 | City of Columbia · Columbia | State and local governments | 39 | $56.6 million |
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| 65 | City of Joplin · Joplin | State and local governments | 50 | $54.1 million |
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| 66 | American Soybean Association · Saint Louis | Nonprofits | 3 | $53.6 million |
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| 67 | Southeast Missouri State University · Cape Girardeau | Colleges and universities | 19 | $52.8 million |
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| 68 | Extension Foundation · Kansas City | Nonprofits | 11 | $51.6 million |
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| 69 | Big Springs Medical Association Inc. · Ellington | Other | 21 | $50.6 million |
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| 70 | Department of Public Safety Missouri · Jefferson City | State and local governments | 42 | $49.7 million |
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| 71 | Terminal Railroad Association of St. Louis · Saint Louis | Businesses | 2 | $48.8 million |
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| 72 | City of Springfield · Springfield | State and local governments | 34 | $48.2 million |
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| 73 | Central Missouri Community Action · Columbia | Nonprofits | 13 | $48.1 million |
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| 74 | The Empire District Electric Company · Joplin | Businesses | 1 | $47.5 million |
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| 75 | Union Electric Company · Saint Louis | Businesses | 1 | $47.1 million |
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| 76 | City of Springfield, Missouri · Springfield | State and local governments | 17 | $44.1 million |
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| 77 | St Louis Children's Hospital · Saint Louis | Other | 3 | $43.0 million |
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| 78 | Douglass Community Services, Inc · Hannibal | Nonprofits | 10 | $41.9 million |
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| 79 | Total Highspeed LLC · Nixa | Other | 3 | $41.1 million |
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| 80 | Knob Noster School District R 8 · Knob Noster | State and local governments | 9 | $41.1 million |
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| 81 | Junior College Dist Newton-MC · Neosho | State and local governments | 31 | $40.0 million |
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| 82 | South Central Missouri Community Action Agency · Winona | Nonprofits | 4 | $39.5 million |
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| 83 | University of Central Missouri · Warrensburg | Colleges and universities | 14 | $38.7 million |
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| 84 | Samuel U. Rodgers Health Center, Inc · Kansas City | Nonprofits | 9 | $38.4 million |
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| 85 | St. Louis, City of · Saint Louis | State and local governments | 106 | $38.0 million |
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| 86 | Northwest Health Services Inc · Saint Joseph | Nonprofits | 11 | $38.0 million |
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| 87 | Betty Jean Kerr People's Health Centers · Saint Louis | Nonprofits | 8 | $37.6 million |
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| 88 | Missouri Coalition for Primary Health Care · Jefferson City | Nonprofits | 15 | $37.5 million |
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| 89 | Carestl Health · Saint Louis | Other | 10 | $37.4 million |
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| 90 | Ozark Tri-County Health Care Consortium · Neosho | Other | 8 | $36.4 million |
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| 91 | Community Action Partnership of Greater St. Joseph · Saint Joseph | Nonprofits | 3 | $36.3 million |
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| 92 | Missouri Family Health Council, Inc · Jefferson City | Nonprofits | 3 | $35.7 million |
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| 93 | Missouri Department of Agriculture · Jefferson City | State and local governments | 97 | $35.6 million |
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| 94 | Missouri Southern State University · Joplin | Colleges and universities | 11 | $32.5 million |
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| 95 | Preferred Family Healthcare, Incorporated · Kirksville | Nonprofits | 25 | $32.1 million |
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| 96 | Columbia College · Columbia | Colleges and universities | 6 | $32.1 million |
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| 97 | Jefferson Franklin Community Action Corporation · Hillsboro | Nonprofits | 12 | $31.9 million |
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| 98 | City of St. Louis Department of Health · Saint Louis | State and local governments | 4 | $31.6 million |
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| 99 | East Missouri Action Agency, Inc. · Park Hills | Nonprofits | 7 | $31.2 million |
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| 100 | Center for Human Services · Sedalia | Nonprofits | 5 | $30.9 million |
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