# Molecular Tools for Management of Threatened and Endangered Species

Source: https://welltoldgrants.com/grants/w81ewf-22-soi-0009-molecular-tools-for-management-of-threatened-and-endangered/  
Official notice: https://www.grants.gov/search-results-detail/337653

> Dept. of the Army -- Corps of Engineers offered grants of up to $64,300 to the applicants described in the notice. It expected to make one award worth $64,300 in total. Applications closed on March 18, 2022.

| Field | Value |
|---|---|
| Opportunity number | W81EWF-22-SOI-0009 |
| Agency | Dept. of the Army -- Corps of Engineers |
| Status | Forecast |
| Deadline | March 18, 2022 |
| Award ceiling | $64,300 |
| Award floor | not stated |
| Expected awards | 1 |
| Total funding | $64,300 |
| Cost sharing | not required |
| Assistance Listings | 12.630 |

## Who can apply

- Others (see text field entitled "Additional Information on Eligibility" for clarification)

This opportunity is restricted to non-federal partners of the Great River and Great Lakes – Northern Forest Cooperative Ecosystems Studies Unit (CESU).

## What the grant funds

Background: The government seeks research and technical support for the development and demonstration of molecular tools to assess at-risk species occurrence, genomics, and disease risk on military installations from which samples were collected (Fort McCoy, WI, and Camp Grayling, MI). Exact analyses conducted and the need for additional sample collection will be decided based upon input from installation, ERDC-CERL, and CESU partners. It is anticipated that results from these analyses will be relevant for management throughout the focal species ranges. Details of primary tasks are as follows: Task 1: DNA metabarcoding to assess freshwater aquatic communities Fort McCoy, WI comprises 4,400 acres of wetlands (NRCT, 2015) that harbor diverse communities, including a number of at-risk species such as wood turtle, Glyptemys insculpta, Blanding’s turtle, Emydoidea blandingii, and four amphibian species considered to be either state endangered (i.e., Blanchard’s cricket frog, Acris blanchardi) or species of special concern (i.e., the four-toed salamander, Hemidactylium scutatum; the pickerel frog, Lithobates palustris; and the northern leopard frog, Lithobates pipiens). In addition, Wisconsin Department of Natural Resources currently regulates 99 aquatic invasive species (64 animals and 35 plants) that pose a risk to Fort McCoy’s freshwater ecosystems. Understanding where these at-risk and invasive species occur across the installation is a first step in determining effective management strategies. Ponds, ephemeral pools, and streams throughout Fort McCoy will be sampled at three time periods (spring, summer, and fall) during 2022. Specific sampling locations and timing will be determined in consultation with CERL and Fort McCoy Natural Resources Branch staff, based on the natural history of focal species, historical records, and availability of suitable habitat. Sampling estimates include 10 – 30 locations per sampling period with three replicate water samples collected in 1-liter bottles at each location and stored on ice. Sampling effort will include a 1-liter bottle of molecular-grade water at each site to serve as a control. Water samples will be filtered using a vacuum pump through 0.80 μm cellulose nitrate filter. Filters will be stored in vials of cetyl trimethylammonium bromide (CTAB) buffer until DNA extraction.

## Questions people ask

### Is W81EWF-22-SOI-0009 still open?

No. It closed on March 18, 2022. A newer notice is open: W912HZ26S0001, closes January 1, 2027 (55 days left).

### Who can apply for Molecular Tools for Management of Threatened and Endangered Species?

Dept. of the Army -- Corps of Engineers accepted applications from the applicants described in the notice. The notice adds further conditions, so read its eligibility section.

### What is the deadline for Molecular Tools for Management of Threatened and Endangered Species?

Closed March 18, 2022. Applications are no longer accepted; the program's newer notices are on this page when there are any.

### How much funding is available?

Awards of up to $64,300 each. About $64,300 in total for one award.

### Who has won Basic, Applied, and Advanced Research in Science and Engineering grants before?

The largest recipients over five fiscal years were Battelle Memorial Institute, Msi Stem Research & Development Consortium, University of California San Diego. The program made 1,101 awards worth $1.3 billion in that time.