# Understanding Adverse Effects of Per- and Polyfluoroalkyl Substances on Nontarget Species

Source: https://welltoldgrants.com/grants/w81ewf-21-soi-0025-understanding-adverse-effects-of-per-and-polyfluoroalkyl/  
Official notice: https://www.grants.gov/search-results-detail/334545

> Engineer Research and Development Center offered grants of up to $300,000 to the applicants described in the notice. It expected to make one award worth $300,000 in total. Applications closed on September 20, 2021.

| Field | Value |
|---|---|
| Opportunity number | W81EWF-21-SOI-0025 |
| Agency | Engineer Research and Development Center |
| Status | Forecast |
| Deadline | September 20, 2021 |
| Award ceiling | $300,000 |
| Award floor | not stated |
| Expected awards | 1 |
| Total funding | $300,000 |
| Cost sharing | not required |
| Assistance Listings | 12.630 |

## Who can apply

- Others (see text field entitled "Additional Information on Eligibility" for clarification)

This opportunity is restricted to non-federal partners of the Gulf Coast Cooperative Ecosystems Studies Unit (CESU).

## What the grant funds

Background: Per- and polyfluoroalkyl substances (PFAS) have been released into the environment primarily through the use of aqueous film-forming foams that have been used to fight flammable liquid pool fires, but also through the ubiquitous use and disposal of a multitude of industrial and consumer-based products that contain PFAS. The film-forming foams are complex mixtures normally containing fluorinated- and hydrogenated-surfactants that are used to extinguish fires involving highly flammable liquids. PFAS have become an important emerging concern worldwide, as these substances are found in biota, water and sediments and constitute potential hazards to environmental and human health. They are known to cause immunotoxicity and alter lipid metabolism, among other toxicological endpoints. To better understand the potential health hazards of PFAS and related compounds, molecular endpoints such as gene expression are being analyzed and incorporated into the Adverse Outcome Pathway (AOP) framework. Changes in gene expression, or transcriptomics, are a powerful measurement of chemical effects as it measures changes in thousands of genes at once. However, understanding how the molecular endpoints relate to apical toxicity and health hazard remains a challenge. Brief Description of Anticipated Work: Required Work Objectives: This project will determine changes in transcriptomes and behavior that can be linked to PFAS exposures from both field and laboratory experiments. The project will also address the impact of PFAS on biodiversity in the environment using molecular genetic approaches to identify species in water and sediment samples. The project is focused on determining health effects zebrafish and on metagenomic approaches to monitor changes in the abundance of different species in environmental samples. The ultimate goal of this effort is to develop methods that accelerate our understanding of the adverse effects of PFAS on aquatic species in order to inform risk assessment. Objective 1: RNA sequencing analysis of zebrafish embryo. We will receive fish samples from EL scientists and will perform RNAseq on the tissues of interest as discussed. Samples will be run on either HiSeq or NovaSeq Illumina platforms, depending on the number of samples and coverage needed. Bioinformatics analysis will be performed on the sequencing data in order to identify differentially expressed genes, enriched pathways, or other endpoints of interest.

## Questions people ask

### Is W81EWF-21-SOI-0025 still open?

No. It closed on September 20, 2021. A newer notice is open: W912HZ26S0001, closes January 1, 2027 (55 days left).

### Who can apply for Understanding Adverse Effects of Per- and Polyfluoroalkyl Substances on Nontarget Species?

Engineer Research and Development Center accepted applications from the applicants described in the notice. The notice adds further conditions, so read its eligibility section.

### What is the deadline for Understanding Adverse Effects of Per- and Polyfluoroalkyl Substances on Nontarget Species?

Closed September 20, 2021. Applications are no longer accepted; the program's newer notices are on this page when there are any.

### How much funding is available?

Awards of up to $300,000 each. About $300,000 in total for one award.

### Who has won Basic, Applied, and Advanced Research in Science and Engineering grants before?

The largest recipients over five fiscal years were Battelle Memorial Institute, Msi Stem Research & Development Consortium, University of California San Diego. The program made 1,101 awards worth $1.3 billion in that time.